A 2.00-mL solution of apotransferrin was titrated as illustrated in Figure 17-10. It required 163 L of

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A 2.00-mL solution of apotransferrin was titrated as illustrated in Figure 17-10. It required 163 L of 1.43 mM ferric nitrilotriacetateto reach the end point.

(a) Why does the slope of the absorbance-versus-volume graph change abruptly at the equivalence point?

(b) How many moles of Fe(III) (= ferric nitrilotriacetate) were required to reach the end point?

(c) Each apotransferrin molecule binds two ferric ions. Find the concentration of apotransferrin in the 2.00-mL solution.


Figure 17-10

End point 0.400 0.300 0.200 0.100 100 200 300 ul of Fe(lll) added Corrected absorbance at 465 nm

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