Equation 30-19 can be rearranged to produce the Equation Where vmax 5 k2[E]0, the maximum velocity when

Question:

Equation 30-19 can be rearranged to produce the Equation

Where vmax 5 k2[E]0, the maximum velocity when [S] is large.
(a) Suggest a way to use this equation in the construction of a calibration (working) curve for the enzymatic determination of substrate.
(b) Describe how the resulting working curve can be used to find Km and vmax.