Two proteins A and B are chromatographed through a bed. The total volume of the bed is 100 L and void fraction is 0.30. The equilibrium constants K are 10.0 and 12. 0 for A and B, respectively. Estimate the volume of eluate at which the peak will occur for each of these materials? When 600 and 700 L of eluate is collected the percentage of A was 2.0 and 16. 0 respectively. Determine the quantity of eluate that must be collected to obtain a yield of 95% of A. Assume σ for the both solutes is the same.