The Frster theory of resonance energy transfer and the basis for the FRET technique can be tested

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The Förster theory of resonance energy transfer and the basis for the FRET technique can be tested by performing fluorescence measurements on a series of compounds in which an energy donor and an energy acceptor are covalently linked by a rigid molecular linker of variable and known length. L. Stryer and R.P. Haugland (Proc. Natl. Acad. Sci. USA 58, 719 (1967)) collected the following data on a family of compounds with the general composition dansyl-(l-prolyl)n-naphthyl, in which the distance R between the naphthyl donor and the dansyl acceptor was varied from 1.2nm to 4.6nm by increasing the number of prolyl units in the linker:

Are the data described adequately by eqn 20G.10? If so, what is the value of R0 for the naphthyl–dansyl pair?


Data in eqn 20G.10 

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