Two mutant strains of E. coli each have a temperature sensitive mutation in a gene that encodes
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Two mutant strains of E. coli each have a temperature sensitive mutation in a gene that encodes a product required for chromosome duplication. Both strains replicate their DNA and divide normally at 25°C but are unable to replicate their DNA or divide at 42°C. When cells of one strain are shifted from growth at 25°C to growth at 42°C, DNA synthesis stops immediately. When cells of the other strain are subjected to the same temperature shift, DNA synthesis continues, albeit at a decreasing rate, for about a half hour. What can you conclude about the functions of the products of these two genes?
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Related Book For
Principles of Genetics
ISBN: 978-1119142287
7th edition
Authors: D. Peter Snustad, Michael J. Simmons
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