Figure 5-9 is a chromatogram of component XX and internal standard SS. The small peak early in the chromatogram is an unretained component.
a. What is the linear velocity if the column length is 100 mm? 100 mm?
b. What are the retention time, adjusted retention time, and retention factor for XX and S? S?
c. What is the separation factor between XX and S? S?

Figure 5-9

Transcribed Image Text:

Detector signal 0 X 1 2 Time (min) Harris/Lucy, Quantitative Chemical Analysis, 10e, 2020 W. H. Freeman and Company S 3 FIGURE 5-9 Chromatographic separation of unknown (X) and internal standard (S). A known amount of S was added to the unknown. The relative areas of the signals from X and S allow us to find out how much X is in the mixture. It is necessary first to measure the relative response of the detector to each compound.