Forster resonance energy transfer. Spectra of six dyes are shown here. Select a suitable donor/acceptor pair for each analytical objective.

a. Excitation at 488 nm 488 nm results in little fluorescence at 600 nm 600 nm when the dyes are apart and strong fluorescence at 600 nm 600 nm when they are close.
b. Excitation at 488 nm 488 nm results in no fluorescence when the dyes are close and strong fluorescence at 520 nm 520 nm when they are apart.
c. Excitation at 532 nm 532 nm results in strong fluorescence at 670 nm 670 nm when the dyes are close, and the fluorescence shifts to 570 nm 570 nm when the dyes are apart.

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Ex 350 350 Em 350 Alexa Fluor 405 450 550 650 Wavelength (nm) Ex Em 450 550 Wavelength (nm) WELL Alexa Fluor QSY 9 488 650 750 350 Ex Em 750 350 450 550 Alexa Fluor 555 450 550 650 Wavelength (nm) Alexa Fluor 647 750 350 Wavelength (nm) 450 Ex Abs OSY 21 650 750 650 550 Wavelength (nm) Em Abs 450 550 650 Wavelength (nm) 750 750