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1. (15 points) A chromatographic separation of a two component samples on a 50 cm column gave the retention times for the solutes A and

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1. (15 points) A chromatographic separation of a two component samples on a 50 cm column gave the retention times for the solutes A and B as 2.5 and 3.1 minutes with base widths of the two chromatographic peaks being 0.24 and 0.3 minutes respectively. Calculate the (a) number of theoretical plates, (b) plate height (you may use an average N value for this calculation), and (c) resolution of the two peaks, and (d) resolution of the two peaks if the column length is increased to 80 cm (you may assume that u,k,and H do not change when L is changed)

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