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1. Conversion of glucose to fructose, used as sweetener in the food industry, is catalyzed by glucose isomerase produced by Bacillus coagulans. It is desired
1. Conversion of glucose to fructose, used as sweetener in the food industry, is catalyzed by glucose isomerase produced by Bacillus coagulans. It is desired to culture this bacteria in a chemostat of volume 1 L. Data for the process is shown below. Concentration (g/L) Flow rate Glucose Bacillus (mL/hr) Fructose Concentration Concentration Concentration 30 12 14 1.1 26 9 17 1.3 23 7 19 1.5 20 3 20 1.7 a. What flowrate should be maintained in the chemostat to prevent wash out of cells? Assume a sterile feed and an inlet substrate concentration of 40 g/L. b. What would be the cell and substrate concentration if the flowrate is set to (a)
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