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1. Describe how plasmid pBR322 is used as a cloning vector. What are its special features? 2. For gene-cloning experiments, why is the cleaved
1. Describe how plasmid pBR322 is used as a cloning vector. What are its special features? 2. For gene-cloning experiments, why is the cleaved plasmid DNA often treated with alkaline phosphatase prior to the liga- tion step 3. What are type Il restriction endonucleases? Why are they important for recombinant DNA technology.
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1 Features of pBR322 pBR322 is a doublestranded circular DNA mainly found in E coli Without the help of chromosomal DNA it can replicate by itself The ...
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Microbiology A Systems Approach
Authors: Marjorie Kelly Cowan
4th edition
978-007340243, 73402435, 978-0073402437
