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1. Describe the processes of transformation and conjugation. Why did we not directly transform the plasmids into F.johnsoniae UW102-90? (1) 2. Describe the components of

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1. Describe the processes of transformation and conjugation. Why did we not directly transform the plasmids into F.johnsoniae UW102-90? (1) 2. Describe the components of the PSA11 and PDH 223 plasmids. (1) 3. What gene is mutated in F. johnsoniae UW102-90? How does this experiment confirm that? When answering this question, make sure you define and explain complementation. (1) 4. Explain any sources of error that occurred during your experiment. (1) 1. PSA11 and pDH223 confer erythromycin resistance on F.johnsoniae, but not on E. coli. How would the outcome of this experiment have been different if PSA11 and PDH223 conferred erythromycin resistance on both F. johnsoniae and E. coli? Explain. 44

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