1- Enzymes are only used industrially to make chiral molecules

a) Yes, enzymes are only useful more the manufacture of chiral products such as valuable pharmaceutical intermediates

b) There are no examples of enzymes as catalysts in chemical processes in an industrial setting

c) Enzymes are often used to make chiral products, but can be successfully used in some cases for achiral products

d) Enzymes cannot make chiral products, they are only used to make products which do not possess chirality

3- An IB manufacturing process is always a more sustainable option than a pure chemical process

a) IB processes are always more sustainable than any traditional chemical methods employed in industry

b) IB can never compete with chemical processes which boast higher productivity and better cost efficiency

c) IB is not always, but in some circumstances, can be more sustainable than a chemical process for a given product

d) IB processes are not scalable as manufacturing routes and so cannot be used for the production of bulk or commodity chemicals

5-IB/biocatalysed processes can be used for ...

a) A wide range of functional molecules including pharmaceuticals, personal care products and bulk chemicals

b) Only for the manufacture of biofuels which are more sustainably resourced than current fuels

c) Only for the manufacture of complex high value products like pharmaceuticals

d) Only for foodstuffs such as dietary supplements, nutraceuticals and flavour compounds

6-When screening for a given transformation to design a new process, you need to start with a hit from a known enzyme on the given substrate of choice

a) An enzyme evolution study needs to start with really simple models

b) If screening commercially available enzymes does not produce a hit on the substrate of choice, then it is not possible to develop an IB process

c) No, in silico design, modelling understanding the reactivity of related simple substrates and can help us design a bespoke enzyme

d) Yes, this is correct

7.-Enzymes that use complex and expensive co-factors like NADPH or PLP cannot be used in economically viable commercial processes.

a) We can add stoichiometric co-factors as this is very cost efficient

b) A number of different ways are known to recycle co-factors like NADH, and these can be scaled up to give viable large scale processes

c) Yes, this is generally true as they cannot be sourced sustainably or regenerated during the reaction

d) Enzymes that use co-factors are generally not used in IB processes as they do not perform synthetically useful reactions of interest to industry

8. When designing a manufacturing process, safe operation is a top consideration

a) Most enzymes work at high pressures so safety needs to be a high priority

b) Enzymes work at very low temperatures and so cooling systems are often required

c) All enzyme reactions are endothermic and this must be taken into account in the design of all IB processes

10. Enzymes are very useful catalysts but only for natural substrates

a) Yes, this is true

b) Enzyme mutants can now be quickly evolved to accept a wide range of unnatural substrates

c) It is not possible to change the substrate for a given enzyme

d) No, but it takes many years to evolve an enzyme for a given substrate