1. For feed media to a bioreactor, the dissolved oxygen concentration is proportional to that in the air surrounding the medium. The oxygen concentration will reach an equilibrium between the medium and the surrounding air. At 37 C and 1.0 atm of pressure, air is 21.2% O, 0.15 % CO and 78.65 % N. a. Determine the mass and molar concentrations of each of the gases in the air. b. Find the concentration of dissolved O in the culture medium. Assume that culture medium has the same O solubility properties as water and that the concentration of O in the air is not affected by the transport of oxygen into the medium. The Henry's constant is 5.21 x 104 atm/mole fraction of O in water. c. Prior to use, the medium is most often stored at 4 C where Henry's constant is 2.95 x104. How will this temperature change affect the concentration of dissolved oxygen in the medium? Show the calculations that support your answer.