Question
1. If your spectrophotometer can measure an absorbance up to1.5, what is the maximum concentration of NADH that you can measurewithout diluting? ? 340 NADH
1. If your spectrophotometer can measure an absorbance up to1.5, what is the maximum concentration of NADH that you can measurewithout diluting? ?340NADH = 6220M-1cm-1. Assume a pathlength of 1 cm.For this one I got 2.41e-4 M. Please confirm theanswer, show all steps neatly and clearly.
2. If you add 3 mL of water to 1 mL of NADH, mix and get anabsorbance of 0.2, what is the concentration of the originalNADH? Assume a pathlength of 1 cm. For this answer I got1.28e-4. Please confirm the answer, show all stepsneatly and clearly.
3. What size test tubes will you use to mix the reagents for theBradford protein assay? For this answer I got 13x100mm, please show all work neatly and clearly. Thisis the question I need answered. Thank you in advance
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Answer Q1Given data Max Absorbance 15 Molar Absorption Coefficients e of NADH at 340 nm 6220 1MCm pa...
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Fundamentals of biochemistry Life at the Molecular Level
Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt
4th edition
470547847, 978-0470547847
