1. In a eukaryotic cell a. Where does transcription take place? b. What polymer is synthesized during transcription? C. What monomers are used to construct this polymer? d. What is the base-pair rule for a DNA strand matching an RNA strand? e. The DNA strand that contains the "blueprint" for the pre-mRNA is called as f. During transcription, in which direction is the DNA molecule read? e. During transcription, in which direction is the pre-mRNA molecule constructed? 2. Consider that the figure shown below is of a pre-mRNA formed in a eukaryotic cell. Draw and list the RNA processing modifications that will occur to this pre-mRNA. (6 points) 5' Exon 1 Intron 1 Exon 2 Intron 2 Exon 3 Intron 3 3' Use this table to answer the question 3-5 First mRNA base (5' end of codon) U 3 UUU U Second mRNA base UCU Phe UUC (F) UCC Ser UUA Leu UCA (S) UUG (L) UCG e A G UAU Tyr UGU Cys UAC M UGC (C) UAA Stop UGA Stop 50 UAG Stop UGG Trp (W) G CAU His CGU CAC HCGC Arg CAA Gin CGA (R) CAG (Q) CGG AAU Asn AGU Ser AACN) AGC (5) CUU CCU CUC Leu CCC Pro CA U CCA (P) CUG CCG AUU ACU AUC Te ACC (D) Thr AUA ACA (T AAA AUG Me ACG GUUT GCU GAU G GUA GUG GUC Val GCC Ala M GAC (D) GGC Gly GCA (A GCG GAA Glu GGA GAG (E) GGG (G) AGA Lys Arg AAG (K) AGG (R) GGU Asp 5 CACUCAGUTAG Third mRNA base (3' end of codon) 3. Consider this DNA template sequence, CAC TAC GGA CTC CTC TTC ACT. The underlined region is an intron. What is its a. Pre-mRNA sequence: b.mRNA sequence: (4 points) c. Anticodons that would be used in the tRNA d. Amino acid sequence 4. The template strand of a gene includes this sequence: 3'-TACTTGTCCGATATC-5'. (3 points) a. What is the sequence of the non-template /coding strand? b. What is the mRNA sequence? c. What is the amino acid sequence? Chapter 14: Transcription and Translation Name: 5. Now consider the effect if the template strand of a gene mentioned in question 4 is mutated to: 3-TACTTGTCCAATATC-5' (3 points) a. Now what is the mRNA sequence? 5. Now consider the effect if the template strand of a gene mentioned in question 4 is mutated to: 3'-TACTTGTCCAATATC-5' (3 points) a. Now what is the mRNA sequence? b. What is the amino acid sequence? c. What type of mutation has occurred? What is the effect of the mutation on the amino acid sequence? 6. In prokaryotic cells, translation begins before transcription is finished. Why is this not possible in eukaryotic cells. (2 points)