$1.$ Prepare stock solution of $500$ppm vitamin E$.(0\mathrm{.}05$g$/100$ml ethanol$).$

$2.$ Prepare six standard solutions from the stock solution.

i$.0\mathrm{.}00$ppm $($blank ethanol$)$

ii$.150$ppm $($use this solution to find $\backslash$lambda max$)$

iii. $75$ppm

iv$.37\mathrm{.}5$ppm

v$.18\mathrm{.}75$ppm

vi$.9\mathrm{.}375$ppm

$*$ unknown $($get from lab assistant$)$

$*1$ppm of vitamin E $($detection limit$).$

$3.$ Fix $\backslash$lambda max. Plot a calibration graph

$($from graph determine unknown and detection limit $1$ppm$.)$