1. Staphylococcus aureus:

A. Confirm with a Gram stain that the organism in question is a Gram-positive cocci in clusters by performing the procedure.

B. Carry out a catalase test to ascertain whether or not the organism in question belongs to the genus Staphylococcus (positive catalase test).

C. Carry out a coagulase test to differentiate between coagulase-positive Staphylococcus aureus and coagulase-negative Staphylococcus (CONS) species. This will require a positive coagulase test.

D. If the organism is CONS, you should do a Novobiocin sensitivity test in order to distinguish between S. epidermidis (which is susceptible to novobiocin) and S. saprophyticus (novobiocin-resistant).

2. Enterobacteriaceae NonLactose Fermenters:

A. Confirm that the organism in question is a Gram-negative bacilli by performing a Gram stain on it.

B. Carry out an oxidase test in order to distinguish between bacteria that are oxidase-positive and bacteria that are oxidase-negative.

Enterobacteriaceae are oxidase-negative.

C. Carry out IMViC tests in order to distinguish between the many genera of Enterobacteriaceae (such as Proteus, Salmonella, Shigella, Pseudomonas, and Yersinia).

The indole test (I), the methyl red test (M), the Voges-Proskauer test (V), and the citrate test (C) are all included in the IMViC battery of tests (C).

D. Determine the Enterobacteriaceae species' genus by interpreting the findings of the IMViC tests and coming up with a name for it.

Can you Draw it in diagram