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1. The fatty acids in the triglycerides are derivatized to methyl esters before separation. Why not just hydrolyze the triglycerides and separate the free fatty
1. The fatty acids in the triglycerides are derivatized to methyl esters before separation. Why not just hydrolyze the triglycerides and separate the free fatty acids instead?
2. Triglycerides, methyl esters and fatty acids can also be separated by liquid chromatography. What problems could arise if one tried to perform the analysis in this laboratory with LC?
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