1. The following figure shows a DNA digested with restriction enzymes. The lane A shows the DNA marker (or control) and the others the digested DNA. Which are the two lanes where the restriction enzyme reaction was not able to cut the DNA substrate and the DNA results uncut? Write your answer in the blank space below: A B C D E If a 2. The fragment below is cut by two enzymes, EcoRI and BamHI. on their specific recognition sites. How many fragments do you anticipate detecting in an agarose gel run if you cut the fragment with both enzymes at the same time? Which is the size of the fragment (number of nucleotides) that will run on the bottom of the gel? mutation will change one recognition site (Red arrow) for the enzyme EcoRI, making it not cutting at that particular site. how many fragments you will detect on a gel run? EcorRI 200 1000 BamHI BamHI EcorRI 150 Number of base pairs 250 245 3. In the gel below the lane A represents the DNA marker with known sizes (Starting from the upper rectangle we have; 2000bp/10mm, 1500bp/15mm, 1000bp/20mm and 200bp/70mm). How many base pair is the band marked with the red arrow, assuming it ran for 43 mm from the red starting line? (use the graph below, the range MUST be the smallest you can define. Bigger ranges will be considered incorrect) A B C D E 3 Number of base pairs 2000 1750 1500 1250 1000 750 500 250 0 0 10 20 30 40 50 60 70 millimiters