1. The standard recipe for LB Agar plate is to dissolve 40 g of Miller LB Agar in 1 L of water. If you are taking 75 mL of water, what should be the mass of LB agar? Need to show calculations.

2. You wanted to make 5 mL of 100 mg/mL ampicillin stock solution. Calculate the amount of ampicillin needed. Need to show calculations.

3. The concentration of ampicillin in the LB agar medium is 100 microgram/mL. Calculate the volume of the ampicillin stock solution you need to add to make 50 mL of LB agar. Need to show calculations

4. Why do you cool the LB Agar medium before adding the antibiotic?

5. Why do you add ampicillin in the LB Agar medium?