1. Using a cationic, anion exchange column at pH 7.0, which amino acid would be the last to leave the column? A) Arg b) Lys c) Ala d) Asp 2. Which does SDS-PAGE not do? A) trap proteins in SDS micelles b) denature proteins c) make all proteins negatively charged d) break proteins into amino acids 3. The Edman method is used for what? A) locating proteins in cells b) determining sequence of proteins c) generating 3-D images of proteins d) separating proteins based on molar mass 4. The analytical instrument used in conjunction with the Edman method is: a) gel electrophoresis apparatus b) NMR spectrometer c) high pressure liquid chromatograph d) mass spectrometer 5. X-ray crystallography is used for what? A) locating proteins in cells b) determining sequence of proteins c) generating 3-D images of proteins d) separating proteins based on molar mass 6. Fluorescence microscopy is used for what? A) locating proteins in cells b) determining sequence of proteins c) generating 3-D images of proteins d) separating proteins based on molar mass 7. NOESY is an analytical technique which involves which instrument? A) gel electrophoresis apparatus b) NMR spectrometer c) high pressure liquid chromatograph d) mass spectrometer 8. MALDI is an analytical technique which involves which instrument? A) gel electrophoresis apparatus b) NMR spectrometer c) high pressure liquid chromatograph d) mass spectrometer 9. ELISA is an analytical technique which uses which instrument? A) microplate reader b) gel electrophoresis apparatus c) fluorescence microscope d) ultracentrifuge 10. Western blotting is an analytical technique which uses which instrument? A) microplate reader b) gel electrophoresis apparatus c) fluorescence microscope d) ultracentrifuge 11. If you wish to identify a specific protein from a mixture of proteins seen on an SDS-PAGE gel, the best method to use would be: a) NMR b) mass spectrometry c) ELISA d) Western blotting 12. If you wish to develop a quantitative assay for a specific protein, the best method to use is: a) NMR b) mass spectrometry c) ELISA d) Western blotting 13. If you wish to generate a 3-D image of a protein as it exists in aqueous solution, the best method is: a) NMR b) mass spectrometry C) ELISA d) Western blotting 14. If you wish to determine the exact molar mass of a protein, the best method is: a) NMR b) mass spectrometry c) ELISA d) Western blotting 15. If you wish to determine which cellular region contains the highest concentration of a specific protein, the best method to use is: a) differential centrifugation b) analytic microtomography c) microparticle analysis d) fluorescence microscopy
