1. What happens in automated Sanger DNA sequencing reaction if you forget to add the following? Write 2-3 lines of answer for each one, providing full justification. a) all fluorescent ddNTPs? b) fluorescent ddGTP? c) all dNTPs? d) dGTP?

2. Modifications of histone proteins affect gene expression by epigenetic changes to the chromatin structure/architecture. How can one study if, for example, monomethylation of the amino acid Lysine (residue 8) of histone H2A affects the expression of gene X in liver tissue, under starvation compared to after eating? Explain the technique that can be applied and its principles, with simple but clear explanations.