Question
1. What types of protein would you not choose to produce in E. coli? Describe in detail two types of bacterial promoter systems and how
1. What types of protein would you not choose to produce in E. coli? Describe in detail two types of bacterial promoter systems and how they have been adapted for recombinant protein production, including both rapid and gentle induction.
2. Describe three factors that affect protein solubility and how they are used in bulk fractionation of proteins.
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Elementary Principles of Chemical Processes
Authors: Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
4th edition
978-1118431221, 9781119192138, 1118431227, 1119192137, 978-1119498759
