1 .What would be the best name for the actor labeled "A"? a. Lab technician b. Data Analysis Software c. Volunteer d. Secondary assistant e. None of the above

2. What would be the most appropriate question for the decision labeled "B"? a. Professor available? b. Worthy of filing? c. Any anomalous results? d. New discovery? e. None of the above

3. What would be the most appropriate description of the process labeled "C"? a. Produces sequences b. Extracts genes c. Removes liquid d. Replicates genes e. None of the above

4. What would be the most appropriate description of the process labeled "D"? a. Runs algorithm b. Conducts data analysis c. Updates file server d. Sends alert to assistant e. None of the above

5. What would be the most appropriate question for the decision labeled "E"? a. Sequencing successful? b. Shift over? c. Any more sequencing to be done? d. Data accurate? e. None of the above

6. What would be the best name for the entity labeled "A"? a. Lab technician b. Supercomputer c. Sequencing machine d. File server e. None of the above

7. What would be the most appropriate description for the relationship labeled "B"? a. Analyzes b. Presents c. Files d. Organizes e. None of the above

8. What would be the most appropriate description for the relationship labeled "C"? a. Organized into b. Processed by c. Cleaned by d. Converted into e. None of the above

9. What would be the most appropriate description for the attribute labeled "D"? a. Expiration date b. DNA Sequences c. Species d. Source e. None of the above

10. What would be the most appropriate description for the relationship labeled "E"? a. Uploads to cloud b. Extracts c. Cleans and trims d. Annotates e. None of the above

Mini-Case - Read the following narrative carefully KEP Catherine is a research assistant at University of Pennsylvania. She and her peers operate a wide variety of instruments that perform DNA sequencing, genotyping, DNA quantification and quality control. Katherine is primarily focuses on DNA sequencing core activities. The DNA sequencing core process starts when she receives an instruction from her Principle investigator to sequence a collection of samples of DNA. She will start the first stage of the see fencing core, that mary preparation. Library preparation entails a few steps. air wanted Then she conducts various lab work at the god of which she derives the "Fran"- consisting of a pool of genes. This concentrated pool of genes is very important, in that it contains the DNA strands that are to be analyzed and sequenced. Katherine then injects the library liquid into a next generation sequencing machine. This machine produces sequences which are subsequently translated into data. Following the machine's work, Katherine checks if there is any more sequencing to be done by her. If there is, she performs her lab work again to collect another set of library. Otherwise, she you soon to the second phase of the process which is data s se in thewhich is data analysis. First. she will retrieve millions of sequences. After that, she proceeds to transform the data using a software so that i can be used and sold - the analysis software cleans and trims the dirty data. The data is converted from a raw and unstructured form to a clean and organized form. After the data has been cleaned, the software conducts data analysis to samples. ta analysis to find out w ut what sequences are in the original Sometimes there will be some unusual findings in the sequence, like gene defects, mutation, deletion in the genes, etc. Regardless, she will present the cleaned data to her Principle Investigator (PI). If there are no anomalous results, the Pl will file the findings and the process ends. If there are any anomalous results, the PI will then determine if it is necessary to further investigate the anomalous results. Sometimes. the PI will decides that the anomalies are raceused man did not sonny of further investigation. so the process ends with him filing the to professors. Upon analysis, if the professor solves the anomaly, he will report back to the PI and the latter will file the findings. Otherwise. the professor will do further analysis and this will continue until anomalies are resolved and the findings can be filed. Swimlane Diagram Research Assistant Professor paiforira lab work the sequencing mis tata into Reports back to P Principle Irivestigator Employer_10 Professor Research Assistant Employee_ to PK Employer _10 Name antact Info antact into Contact Info "Clean" Data Sample Submission DNA Sequences PX Sample Code detitity of DNA "Dirty" Data Library dentity of DNA Data Analysis Genes Code Software Name "A" Serial Number Function Volume Access Code Function Operator ID Access Code Operator Namie Operator its Operator Name