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10) When running column chromatography, the following precautions must be taken to ensure a proper separation: explain why these precautions are so important. (4 pts)
10) When running column chromatography, the following precautions must be taken to ensure a proper separation: explain why these precautions are so important. (4 pts) a) Never allow the silica to run dry (i.e, never allow the level of eluent to drop below the top level of the silica gel) b) Use the minimal amount of solvent to dissolve and load the mixture c) Ensure your eluent system possesses the right polarity. d) Ensure the column and the layers within are perfectly straight and leveled
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