Question
1.A. There is a solution which contains only protein A. You place this solution in the spectrophotometer and measure the absorbance at 280nm. The instrument
1.A. There is a solution which contains only protein A. You place this solution in the spectrophotometer and measure the absorbance at 280nm. The instrument gives a reading of 0.4, estimate the concentration of protein A in this solution. (5 points)
1.B. There is a solution which contains only protein B. You place this solution in the spectrophotometer and measure the absorbance at 280nm. The instrument gives a reading of 1.3, estimate the concentration of protein B in this solution. (5 points)
1.C. You accidentally leave solution B out for too long and obtain a cloudy precipitate within the solution. You decide to still read the absorbance using the spectrophotometer. Will your absorbance reading be accurate in this case? Why or why not? (5 points)
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