1-Download/ install and run Prodigal ( a prokaryotic prediction tool/programming Gene finding Algorithm) 2-Test this Prodigal gene finder on a plasmid of the model organism E. coli O157:H7, The test data can be downloaded from GenBank, accession AB011549.2. 3-Compare the gene prediction with the ''official'' annotation on Genbank, 4-And display the results of the comparison as a web page. 5-Write a CGI script to read both the GenBank (reference) annotation and predicted output from Prodigal tool, 6-compare their predicted coordinates and 7-Populate these within an HTML5-compliant template. The output should include summary information at the top, such as: Count of genes in the reference annotation Count of predicted genes Count of genes with exact matching coordinates between reference and prediction (5'' and 3'' agreement) Count of genes with 5'' agreement but 3'' disagreement Count of genes with 5'' disagreement but 3'' agreement Count of genes predicted with no overlap to a reference gene -Table showing all gene coordinates of the reference annotation and, beside each, the corresponding (overlapping) predicted coordinates from Prodigal tool. -Each cell of this table that represents a coordinate from the Prodigal gene predictor should have one of the following class attributes applied to it, ''agrees'' or ''disagrees'', when compared to the reference coordinates. -The output should be like the one in the attached output example.png file just replace the Glimmer3 with this Prodigal gene finder. When performing these comparisons, make sure to: 1-Take into account the strand of the genes involved, else your 5'' and 3'' agreement calculations will be off. 2-Also be sure to use the CDS features within the genbank file for your comparison, as a single gene in prokaryotes can have multiple CDS as children. What you will need is: 1-You will need the GenBank entry, from which you can parse out the gene coordinates, 2-And the FASTA version of the DNA molecule itself to be analyzed using Prodigal gene finder. 3-Run it on the E. coli plasmid, saving the results into HTML file.