1.Extract the Reagent and Product from this Experiment (Pre-Lab Note)

2.Prepare the Flow Chat of Procedure

3. Observation

4. Conclusion

Reagents and General Procedure REAGENTS (all solutions will be prepared for you, available every week): - Alcohol Dehydrogenase (ADH) 0.5mg/mL in 0.05%(w/v) bovine serum albumin, keep on ice. - 1.0mM Nicotinamide adenine dinucleotide (NAD+) - 1.0M Ethanol - 0.2M TRIS buffer (Tris (hydroxymethyl)-aminomethane) (pH9.0):1.21g/50mL, bring pH to 9.0 with 0.1MHCl - Distilled water (d2O) General Enzyme Assay Procedure: This procedure is to be followed every time an enzyme assay is run. The only difference will be in the order of addition and the identity of the reagents. All measurements should be done in duplicate (ideally triplicate if there is time). Zero the spectrophotometer with a cuvette filled with distilled water. Make sure the spectrophotometer is ready to begin the kinetics experiment. The wavelength should be set at 340nm, and to obtain absorbance readings every 5s for 60s. When the spectrophotometer is ready, quickly add your reagent(s) to start reaction (specified in each experiment), cover cuvette with a lid, mix gently by inversion, place cuvette in spectrophotometer and begin assay as soon as possible. Be consistent with your timings for each assay. Note: Final volumes for enzyme assays are always 2500L(2.5mL)