1.Which of the following is true when fixing tissues?

a.It must still be alive

b.It must be cut into thin slices

c.It must be fixed and embedded in a solid medium

d.Both b and c

e.Both a and c

2.Why does cryoelectron microscopy circumvent the limitations oftraditional electron microscopes?

a.Because it avoids artifacts introduced by fixation anddehydration

b.Because it can be used to study live cells

c.Because it facilitates time-lapse experiments

d.Because it uses high-contrast heavy metals to stain thespecimens

3. __________ transmembrane proteins have the _________ in theER lumen and the __________ in the cytosol

a.Type 1, N-Terminus, C-Terminus

b.Type 2, N-Terminus, C-Terminus

c.Type 3, C-Terminus, N-Terminus

d.None of the above