2. Calculate how you world prepare 100 mL of a 0.1 M TRIS-HCI buffer, pH 7.4 using solid TRIS base (tris-(hydroxymethyl)-aminomethane) (MW 121.1. pka 8.08). You may find TRIS under several different names (THAM, Trizma, and Sigma 7-9) and 1 M hydrochloric acid. This is not easy, this is my hint...follow the rules and start with the equation...think about it. (5 pt) 3. What is the new pH of this buffer if we add 1 mL of 1 M HCl to the buffer you made in 2? (3 pt)