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2. Carnobacterium are a genus of bacteria that are found in the ocean and in many food products. A strain (#1) of this bacteria

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2. Carnobacterium are a genus of bacteria that are found in the ocean and in many food products. A strain (#1) of this bacteria can make its way into a shark's brain and cause brain infection. The infection causes the shark to become disoriented and eventually strand itself on nearby beaches. You want to see whether a new strain (#2) of this hacteria will have the same result on three shark species (thresher sharks - A, salmon sharks - B, and leopard sharks - C). You expose four sharks in each species to the bacterial strain known to cause the infection, and four other sharks in each species to the new bacterial strain. After three weeks, you sacrifice the sharks and record how much (%) of the shark's brains has been infected by the bacteria. Shark Species Bacterial A B Strain 60.5 59.1 42.9 56.1 39.5 45.5 #1 55.9 42.9 58.4 44.5 42.4 47.1 41 63.8 60.7 52 62.3 56.3 #2 41 49.7 57.9 45 50.4 68.9 a. Was a pretest performed? If so, fill in the values in the table. If not, explain why. (1 pt) Test type Ho HA Crit/Cale or exact p-value b. What was the conclusion of your pre-test? Do you need to transform your data? If so, what transformation did you use and what was your new p-value or critical/calculated values? (1 pt) Transformation New Cale/Crit or p-value c. What are all of your null and alternative hypotheses for your main test? (2 pt) d. (+mplete the ANOVA table (3 pt): Variance source Bacterial Strain Shark Species Interaction Within Total SS df MS F P-value c. What conclusions can you draw at this point? Do you need to do any post-hoc testing within the bacterial strain or shark species treatments? (1 pt) f. Complete the interaction plots below. Create a legend under each of your plots. (3 pt) 60 60 55 50 50 49 45 40 55 50 45 40 35 35 A B C 1 2 g. Based on the results of your ANOVA any post-hoc testing, and your interaction plots, what is your biological conclusion? Be as specific as possible. (1 pt)

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