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2. Plot the following Lineweaver-Burke plot to be able to answer the following questions: i) Identify the type of inhibitor which affects this reaction. ii)

2. Plot the following Lineweaver-Burke plot to be able to answer the following questions:

i) Identify the type of inhibitor which affects this reaction.

ii) Calculate the Vmax and KM for the uninhibited enzyme.

iii) Estimate the Kcat for this enzyme and comment on the efficiency of its reaction.

3. Compare and contrast the differences in the Lineweaver-Burke plot for an uncompetitive- and non-competitive inhibitors.

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