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2. Students at the LSSU developed a spectrophotometric method for the simultaneous determination of acetylsalicylic acid (ASA), acetaminophen (ACH) and caffeine (CAF) using absorbance at
2. Students at the LSSU developed a spectrophotometric method for the simultaneous determination of acetylsalicylic acid (ASA), acetaminophen (ACH) and caffeine (CAF) using absorbance at 228,243 , and 273nm with a 1.0cm cuvette. (a) What material should the cuvette be made of? (b) Why did the students pick 228,243 , and 273nm for their analysis? CHEM 332 (c) In developing the procedure, the students sought concentrations that gave absorbance readings between 0.3 and 1.2. Why? (d) Determination of unbuffered mixtures resulted in errors up 7%. Acidification of the standards and mixtures resulted in less than 2.5% What was cause of the initial inaccuracy
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