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2. You just re-suspended your purified Actin-GFP Plasmid with 500 pil of TE Buffer. You add 1ul of your purified Actin-GFP Plasmid to a NanoDrop
2. You just re-suspended your purified Actin-GFP Plasmid with 500 pil of TE Buffer. You add 1ul of your purified Actin-GFP Plasmid to a NanoDrop Spectrophotometer, and obtain the following Absorbance readings: Abs 230nm--0.0320 Abs 260nm-0.0260 Abs 290nm-0.0800 Abs 280nm-0.0150 a. From the information above, determine the following: i. Plasmid Actin-GFP Concentration ii. Plasmid Actin-GFP Purity abs260/abs280;.026/.015= 1.733| iii. Plasmid Actin-GFP Yield 2. You just re-suspended your purified Actin-GFP Plasmid with 500 pil of TE Buffer. You add 1ul of your purified Actin-GFP Plasmid to a NanoDrop Spectrophotometer, and obtain the following Absorbance readings: Abs 230nm--0.0320 Abs 260nm-0.0260 Abs 290nm-0.0800 Abs 280nm-0.0150 a. From the information above, determine the following: i. Plasmid Actin-GFP Concentration ii. Plasmid Actin-GFP Purity abs260/abs280;.026/.015= 1.733| iii. Plasmid Actin-GFP Yield
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