(20 pts) The decomposition of 1-kestose is a key step in the production of fructo-oligosaccharides, which are found in many health foods because of their non-caloric and non-cariogenic nature. A study was conducted in the decomposition of 1-kestose using -fructosfuranosidase, both in the presence and absence of the inhibitor glucose. a. The following initial rate data were obtained in the absence of glucose:

(20 pts) The decomposition of 1-kestose is a key step in the production of fructooligosaccharides, which are found in many health foods because of their non-caloric and non-cariogenic nature. A study was conducted in the decomposition of 1-kestose using -fructosfuranosidase, both in the presence and absence of the inhibitor glucose. a. The following initial rate data were obtained in the absence of glucose: Determine the maximum rate and Michaelis constant from these data. b. The following initial rate data were obtained in the presence of 100gL1 glucose: Using the values of maximum rate and Michaelis constant from part a), determine the type of inhibition (with explanation for your answer) and estimate the value of the dissociation constant