3 4 3 A laboratory in Japan performed a similar experiment on earthworms from different localities of the Japanese archipelago, but instead of linking trypsin activity within worms to anatomical features, they were interested in geographical differences in the ability of the worms to breakdown environmental pollutants using the enzyme polyphenol oxidase. The protocol was similar to the one you used, except that enzyme activity was measured from 100 ul of worm extract. Data obtained are displayed below: Locality Replicate Amount of product Concentration of total liberated over 10 protein in worm extract minutes (pmol) (ug/ml) Hokkaido 1 101 4 2 87 107 4 88 3 5 89 5 Hon-shu 126 2 120 3 156 153 5 5 119 4 Kyu-shu 103 99 5 94 6 108 3 5 110 5 Okinawa 88 90 96 4 100 5 5 112 3 1 2 3 4 4 4 1 2 3 4 1 2 3 3 4 6 From the data in the table, calculate the specific activity of polyphenol oxidase (in units of umol/min/mg protein) from each locality, presenting the data as mean = standard error (n=5). (25 marks) Locality Mean Specific activity umol/min/mg protein Standard error Hokkaido Hon-shu Kyu-shu Okinawa