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3. An enzyme immobilized on the surfaces of nonporous polystyrene beads. The enzyme is inhibited at high concentrations of substrate. The intrinsic value kinetic parameters

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3. An enzyme immobilized on the surfaces of nonporous polystyrene beads. The enzyme is inhibited at high concentrations of substrate. The intrinsic value kinetic parameters of the enzyme are: Vmax and Km of SDI] mmolesfhfg and 10 mM respectively. The maximum reaction rate attainable is 264 mmolesfhfg. Based on the following, determine; 3.1. The inhibition constant of the enzyme 3.2. If the system becomes diffusion limited, with a Damkoehler number of 5 and a bulk concentration of 10 mM, then estimate the effectiveness factor of the beads

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