3. Pespin is an aspartyl protease. A few peptides were tested for cleavage with pepsin. The data is shown below.

	E total	[S]	Kcat	Km
Peptide 1	10	30-90	0.002	95
Peptide 2	0.0012	23-218	34	60
Peptide 3	0.004	21-110	112	65

i. Give the equation for line in a Lineweaver-Burke plot for peptide 2.

ii. What substrate concentration will be necessary to achieve Vmax for peptide 3?

iii. Which peptide works best with pepsin? Explain.