300 u 100 1 mL culture 100 UL 100 ut 100 UL transfered transferred UUUUUU 900 ut of LB 900 ut of LB 900 uL of LB 900 ut of LB 900 uL of LB Mix well Mix well Mx well Dilution: Mix well 10- Mix well 10 10 104 10-5 Figure A4. Serial dilutions. See text for details. Note that in this example we mixed 100ul of cells with 900uL of cells. Why didn't we do 1ul in 9ul water? Wouldn't this have accomplished the same thing? It might have, but the error would have been higher. Is hard to pipette one microliter even with the most accurate pipette. By mixing 100 with 900 you are less likely to introduce error in the experiment. 100 UL transferred 52 U 900 ut of LB Mix well 106 6. How many times should you serially dilute a 10 cells/uL bacterial culture in order to plate 100 microliters of 10cells/uL? 7. You want to plate 450 cells of yeast on a plate. Your cells are at a concentration of 6.3 X 105 cell/ut. Devise a plan to do this.