Question: 4. (20 points) In this experiment from Ohnishi, et al., total cell lysates (TCL) prepared from embryonic fibroblasts from wild type mice or transgenic

4. (20 points) In this experiment from Ohnishi, et al., total cell

4. (20 points) In this experiment from Ohnishi, et al., total cell lysates (TCL) prepared from embryonic fibroblasts from wild type mice or transgenic mice expressing cagA were subjected to immunoprecipitation (IP) and Western blotting (IB) using the antibodies listed. Describe how the experiment was performed, the controls used and the results obtained. C B6 CAG-cagAHs IP: IgG HA IgG HA IB: anti-HA IB: anti-phospho- tyrosine IB: anti-SHP-2 Immunoprecipitates TCL CAG-cagAHS

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