4. Chromatography Anion exchanger is used to separate a low-level DNA, 20mg/L, from an initial 2.5 L product solution, with an elution flow rate 1L/min. No other components are natively charged at the operating pH for the column. (15 marks) The column information: Void fraction 0.2. Column length 15cm, diameter 40cm. Total site density of the column surface St (first 4 digits of ID) mg/L. Equilibrium constant of the adsorption 3mL/mg. a) Explain whether it is suitable to use Linear equilibrium to model the adsorption here. b) Determine the DNA concentration in the station phase using Langmuir Isotherm. c) Calculate the time span between the peak of DNA and 20% concentration coming out of the column on the elution profile