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4 . Diffusion coefficient for solutes depending on the viscosity of the medium in which they are diffusing. In that regard, does band broadening increase

4 .

Diffusion coefficient for solutes depending on the viscosity of the medium in which they are diffusing. In that regard, does band broadening increase or decrease as the temperature of the column

(

and hence the mobile and stationary phase in it

)

is increased in HPLC

?

Assume the flow rate is above the optimum

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