4. You are attempting to separate a DNA-binding protein (protein X ) from other proteins in a solution. Only three other proteins (A, B, and C) are present. The proteins have the following properties: a. Propose a purification scheme to separate X from the contaminating proteins A,B and C. b. Once you isolate X you perform a number of experiments to determine its sequence. Complete acid hydrolysis to degrade the protein into its constituent amino acids yields the following: E(5),L(4),K(4),S(3),W(2),G(2),C(2),Q(1),A(1),V(1),D(1)I(1),R(1),Y(1),F(1) DNB-treatment to label the N-terminal amino acid yields: DNB-Leu Carboxypeptidase treatment to cleave the C-terminal amino acid yields: E Treatment with the protease trypsin yields: EDYK; IK; SCGE; LWGSSQAK; WEFEELLK; LVCR Treatment with the protease chymotrypsin yields: EF; KW; GSSQAKLVCRIKEDY; EELLKSCGE; LW