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4) You are studying a viral oncogene that induces anchorage-independent growth when expressed in fibroblasts. In order to map the functional domains within that
4) You are studying a viral oncogene that induces anchorage-independent growth when expressed in fibroblasts. In order to map the functional domains within that oncogene, you construct a series of mutants and compare the abilities of the mutants to induce anchorage independent growth with that of the wild type gene. After co-transfecting the wild type or mutant oncogene with an antibiotic resistance marker into fibroblasts and selecting for antibiotic resistant cells, you plate 104 cells in agarose in each of a series of dishes. After allowing time for growth, you obtain the following numbers of colonies per dish. wild type 120 310 440 413 500 mutant 48 15 120 15 23 Do the mutants show differential activity on average compared to the wild-type fibroblasts?
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