5/10 CONFIDENTIAL QUESTION 4: (27 marks) L-Lactate is converted to pyruvate by using lactate dehydrogenase (LDH) enzyme. This study was camied out by using spectrophotometer and oxamic acids as inhibitors. The experimental data is tabulated in Table 2: Table 2: Kinetic data of conversion of L-Lactase to pyruvate Lactate Velocity (MM/min) concentration (mM) No inhibitor 20 mM oxamic 60 m Moxamic acid adid 2.5 1.5 5 5 8 5 25 16 12 7.5 25 55 8.3 15 28 75 95 29 16 8.8 (a) Determine the type of inhibition introduced by different concentration of oxamic acid (13 Marks) (b) Determine the Michaelis Constant, Kv and maximum rate of reaction, for system without inhibitor (4 Marks) (c) Determine the inhibition constants, K, for different concentrations of oxamic acids. (10 Marks)