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6.) 0.1ml of culture was diluted in 9.9ml of sterile water. This is mixed well and further diluted by 2 successive 1/10 dilutions followed by

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6.) 0.1ml of culture was diluted in 9.9ml of sterile water. This is mixed well and further diluted by 2 successive 1/10 dilutions followed by 1/100 dilution. An aliquot of 0.1mL of 1/100 dilution was spread on a plate of nutrient agar. After incubation, 55 colonies were present on this plate. What was the number of colony-forming units /ml of the original culture? (Show all work including successive dilutions, volumes, math, sketch, etc.) lst dilution 0.1ml in 99ml dilution factor 1102 diluted again 1101 and 1102 Final dilution factor 102101102=105 CFu/ml=55105=5.5106(FU/ml)

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