7.)Enzymes that use complex and expensive co-factors like NADPH or PLP cannot be used in economically viable commercial processes.

a) We can add stoichiometric co-factors as this is very cost efficient

b) A number of different ways are known to recycle co-factors like NADH, and these can be scaled up to give viable large scale processes

c) Yes, this is generally true as they cannot be sourced sustainably or regenerated during the reaction

d) Enzymes that use co-factors are generally not used in IB processes as they do not perform synthetically useful reactions of interest to industry

8.) When designing a manufacturing process, safe operation is a top consideration

a) Most enzymes work at high pressures so safety needs to be a high priority

b) Enzymes work at very low temperatures and so cooling systems are often required

c) All enzyme reactions are endothermic and this must be taken into account in the design of all IB processes

d) Most IB processes occur in water at near neutral pH, near ambient temperature, and are not exothermic

9.) Enzymes are only useful for the manipulation of functional groups like ketones and alcohols

a) Enzymes are useful catalysts for the manipulation of functional groups, but also for carbon-carbon and carbon-heteroatom bond synthesis

b) Enzymes are not useful catalysts for functional group manipulation and those that are, are not selective for similar groups at different positions

c) Generally this is true as enzymes do not work on more complex or unactivated chemical groups

d)Enzymes are not useful catalysts for functional group manipulation and are employed mostly in forming bonds between molecules

10.) Enzymes are very useful catalysts but only for natural substrates

a) Yes, this is true

b) Enzyme mutants can now be quickly evolved to accept a wide range of unnatural substrates

c) It is not possible to change the substrate for a given enzyme

d) No, but it takes many years to evolve an enzyme for a given substrate

11.) If you wanted to prepare an ester using an enzyme catalysed process, which enzyme would you use?

a) An Aldolase

b) An oxidoreductase like a ketoreductase

c) An oxidoreductase like an amino acid oxidase

d) A hydrolase like a lipase

12. ) What will a nitrile hydratase transform a nitrile group into?

a) A primary amide only

b) Carboxylic acid only

c) A primary amine only

d) A mixture of products