8. (a). Calculate the predicted G_total at 37C for the below sequence:

5 CGCTGA 3

3 GCGACT 5

b). Note for the above DNA hexamer, there is no symmetry. Thus, the value for G_symmetry is:

9. (a) Calculate H and S for the DNA sequence in question #8. Assume these parameters are calculated analogously to G_total. Express S_total in units of kcal K^-1 mol^-1 (1 e.u. = 1 cal K^-1 mol^-1).

b). You place this DNA hexamer at 60C. What is the new G value? Note that the G values in Table 1 are for 37C only.

TABLE 1 Nearest-neighbor thermodynamic parameters for DNA 'The slash indicates the sequences are given in antiparallel orientation. (e.g., AC/TG means 5-AC-3' is Watson-Crick base paired with 3-TG-5'). The symmetry correction applies to only self-complementary duplexes. The terminal AT penalty is applied for each end of a duplex that has a terminal AT (a duplex with both end closed by AT pairs would have a penalty of +0.1 ikcal /mol for G37 )