8. A lipase is a pancreatic enzyme that degrades fatty acids. In a recent study, Shikha et al. 2017 used calorimetry to explore the impact of immobilizing (or attaching) gold nanoparticles (AuNPs) to the surface of the lipase molecules on their enzymatic activity. Use the authors' results in the figure below to answer the following questions. The lowercase D in Ko represents the deactivation of lipase. Deactivation of the enzyme is considered the forward reaction. 4 Free Lipase 3 A Immobilized lipase . 2 In Ko 1 y=-15931x + 48.46 R2 = 0.894 0 y=-24008x + 70.85 R2 = 0.939 -1 -2 0.0028 0.00285 0.0029 0.00295 0.003 0.00305 0.0031 0.00315 1/T(K-1) a. Estimate the change in enthalpy of deactivation for free lipase. Clearly, explain your reasoning/provide calculations to support your answers. Report your answer in J/mol. (2 points) b. Without doing the math, explain how you could go about obtaining these remaining thermodynamic values for the deactivation of free lipase reaction: Keq, AG, and AS (2 points)