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8.11 For many enzymes, such as chymotrypsin (Case study 8.1), the mechanism of action involves the formation of two intermediates: E+SESESE+SESESSESE+Pv=ka[E][S]v=ka[ES]v=kb[ES]v=kc[ES] Show that the rate

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8.11 For many enzymes, such as chymotrypsin (Case study 8.1), the mechanism of action involves the formation of two intermediates: E+SESESE+SESESSESE+Pv=ka[E][S]v=ka[ES]v=kb[ES]v=kc[ES] Show that the rate of formation of product has the same form as that shown in eqn 8.1, written as: v=1+KM/[S]0vmax but with vmax and KM given by vmax=kb+kckbkc[E]0andKM=ka(kb+kc)kc(ka+kb)

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